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How to make a phylogenetic tree with Bootstrap values in RAxML?

この記事は日本語の記事があります(This article is available in Japanese)
MAFFT→RAxML→FigTreeで遺伝子の系統樹を作成する – Ubuntu19.04

Problem

After analyzing with RAxML, I wanted to make phylogenetic tree with “FigTree” software, and I made it but I could’t make phylogenetic tree with bootstrap values.

Solution

RAxML operation

First of all, you should enter below commands. (If you use raxmlHPC-PTHREADS-AVX and analyzing amino acid sequences. You should change that part of command according to your environment.)

	
$ raxmlHPC-PTHREADS-AVX -f b -m PROTGAMMAAUTO -t RAxML_bestTree.HOGE -z RAxML_bootstrap.HOGE -n BOOTSTRAP

“-f b” option means “drawing bipartition information on a tree”

“-m” means “model”. In this command, I used “AUTO” model for amino acid sequences, so you should choose best model for your data.

“-t” option requires “tree data file”. You may choose “RAxML_bestTree” as ML tree.

“-z” option requires “bootstrap data”. You may choose “RAxML_bootstrap”.

“-n” option means identifier, as you did before.

After enter and execute it, you can find new 3 files in the directory with “.BOOTSTRAP”, and “RAxML_bipartitions.BOOTSTRAP” is object.

How to make phylogenetic tree with FigTree?

You can get FigTree software here.

After execute the application and open “RAxML_bipartitions.BOOTSTRAP” , a dialog will be opened like below.

“入力” means “input”, “取り消し” means “cancel”

You should name “Bootstrap”, and click “OK”.

After loading a phylogenetic tree, see the left side bar, and you can find “Node Labels”. Click the tab, and click the drop-down list and choose “Bootstrap”.

Done!